LOCUS       sequence-77206-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc15_436
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             225..236
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttatttacc ggtgcctttc tgacgcagct gatccagcag cattgcaaaa
       61 cgatccggaa cttcttgaac cagtgcttct tcataaacac gacgcagatt ttcatcaatc
      121 tgctgctgca gttctttacg gcttttacct ttggtgttat caccaccttt tgcggttgcc
      181 tcttgcggac ttgctttcgg ttcggtgctc tgtgccatat ggtatttctc ctctttctct
      241 agtatttatt cgactataac aaaccatttt cttgcgtaaa cctgtacgat cctacaggtc
      301 agccaacgat cgttccgatc agaacaagaa agctatgtgg cctgacgaag cggcgcgcca
      361 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca
      421 gggtggtgaa tatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta
      481 aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac
      541 attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt
      601 caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa
      661 aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat tggaatatat
      721 ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag
      781 gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta
      841 gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga
      901 acataccatt aattgttcct tctctagttg ataattatcg aaaat
//
