LOCUS       sequence-77208-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc16_973
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             660..671
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttaccaacg cagcatacgc ggaccccacc atgcacccag aacggtcgga
       61 accagcatac ccagaacata ccaaacaccc caaaacggaa cacccatttc cggacaatgc
      121 agacaatatg caacggttgc gctgctacct gccagcagac caccggctgc gccagccaga
      181 cgcagacggg tcggtgccag acctttcagt gcccaaaaaa cggtaacaaa tgccggaacg
      241 ctcagcaggg caatattcag tgcacaggta cgccaggtac gaccaaacag cagatctgca
      301 cgtgcatcaa ccggtgcacc cgcaatgcta actgctgcac cacaccaaac cagcagcagc
      361 ggcagaccca gcattttcca acctgctgca cctttaccac ccggacgtgc cagacggctg
      421 gtcagccaca gaccaatcag tgccaggctg gtcggaaatg caactttacc ccaaaacagc
      481 ggtgtctgtg caacttcggt aatatcgcta cgaacaccat acagggtaat ggtcagcagc
      541 agtgctgcgg tcagaccacc cagtgctgca actgccatac gacgaccaac tgcatgacgc
      601 ggaaccggac cttcaccggc tgccagcagt gcaatcagat catcggtttt catatggtat
      661 ttctcctctt tctctagtat ttattcgact ataacaaacc attttcttgc gtaaacctgt
      721 acgatcctac aggtcagcca acgatcgttc cgatcagaac aagaaagcta tgtggcctga
      781 cgaagcggcg cgccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg
      841 aagagagtca attcagggtg gtgaatatga aaaacataaa tgccgacgac acatacagaa
      901 taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc ttatctga
//
