LOCUS       sequence-77209-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc16_3622
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             663..674
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttaccaacg cagcagacgc ggacccagaa cggcacccag cagggtcgga
       61 accagcatac ccagcagata ccacagaccc caaaacggaa cacccatttc cggacaatgc
      121 agacaataaa ccagggttgc ggtgctacca gccagcagac caccggctgc accagcctga
      181 cgcagacggg tcggtgccag accacgcagt gcccaaaaaa ctgcaataaa aaccggtgtg
      241 ctcagcaggg taatattcag tgcacaggta cgccaggtac gaccaaaaat cagatctgca
      301 cgtgcttcag gcggtgcacc aaacaggcta attgctgcac ccagccaaac cagcagcagc
      361 ggaacgccca gcagacccca cagtgcacca ccacgaacac ccggacgtgc cagacgctgg
      421 gtcagccaca gacccagcag ggccaggctg gtcggcagtg caacttttgc ccaaaacagc
      481 ggtgtacgtg caacttctgc cagatcgcta cgaacaccat acagtgcaac ggtcagcagc
      541 agtgcaccca gcagacctgc cagcagtgcc agacccagac gacgtgccag tgcatgacga
      601 tcaaccggac cttctgcggt tgccagcagg ctaatcagtt catcggtttt catcatatgg
      661 tatttctcct ctttctctag tatttattcg actataacaa accattttct tgcgtaaacc
      721 tgtacgatcc tacaggtcag ccaacgatcg ttccgatcag aacaagaaag ctatgtggcc
      781 tgacgaagcg gcgcgccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc
      841 cggaagagag tcaattc
//
