LOCUS       sequence-77210-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc20_992
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             450..461
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttactgttc tgcttcttct gcattaatgc tcagcacaaa ttctgcatca
       61 ctctgatcaa aaacgctcgg cagtgcttct gcgctggtgc tcagcggcag gctcagtgca
      121 actgccagca tacctgctgc aacaccggtc agaccaacac caacccaacc caggcttgcc
      181 agccaacctg cataacggct ccaaaacggt gcaccaggct gctgggctgc tgcaataata
      241 cgctgtgcca gtgccggact cggtgccgga acctgataac gatccagctg gctatccagc
      301 caatgtgcct gacgcagtgc tgcaactgcc tgtgcatcac cacaatccag cagtgcctgt
      361 gctgctgcac gttctgcgct cggccaacgc tgcagatcgg caccataggc atctgccaga
      421 tgaacaaaac gttccggtgt catatggtat ttctcctctt tctctagtat ttattcgact
      481 ataacaaacc attttcttgc gtaaacctgt acgatcctac aggtcagcca acgatcgttc
      541 cgatcagaac aagaaagcta tgtggcctga cgaagcggcg cgccatcgaa tggcgcaaaa
      601 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatatga
      661 aaaacataaa tgccgacgac acatacagaa taattaataa aattaaagct tgtagaagca
      721 ataatgatat taatcaatgc ttatctgata tgactaaaat ggtacattgt gaatattatt
      781 tactcgcgat catttatcct cattctatgg ttaaatctga tatttcaatc ctagataatt
      841 accctaaaaa atggaggcaa tattatgatg acgctaattt aataaaatat gatcctatag
      901 tagattattc taactccaat cattcaccaa ttaattgg
//
