LOCUS       sequence-77211-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc22_1147
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             666..677
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttattcacg ctgaaaacgt gcaatttgtt ccagcagggt ttcggtacgt
       61 gcaacgctac gacctgcatg aacggtatcc agacgttcca gccacggatg accgctacgc
      121 tgcagacgac gataacccag ccaggtgcca ataccaccca gaacaccaac cagcagatta
      181 cacagcagcc atgcatacgg caggctacga ccggtcagtg cacgccatgc tgcatctgca
      241 actgcaaccc acagaaccca aaatgcaaca cccagccacg gtgcaacacg ggtacgccaa
      301 cgacgcagct gtgccagtgc acgctgggtc tgcagcagcg gacgtgcaaa atccagctgg
      361 ctcagcagcc acagctgacg tgctgcgctt gctgccagtg caacacacca aatctgcagc
      421 agaacaccac caacaattgc tgctgcctgt tccggaaatg ccagccagct atgtgcaacc
      481 agaccgctca gggcaattgc acacagcagc tgtgcgctct ggctcagcca cagcggacgc
      541 agatgtgcac gtgctgcatg gctacgcaga tcacccagaa cctgacctgc cagtgctgca
      601 tgctgatcca gacgctgttc caggctacgc catgcaactg caatatcatc cagttccata
      661 tggtatttct cctctttctc tagtatttat tcgactataa caaaccattt tcttgcgtaa
      721 acctgtacga tcctacaggt cagccaacga tcgttccgat cagaacaaga aagctatgtg
      781 gcctgacgaa gcggcgcgcc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc
      841 gcccggaaga gagtcaatt
//
