LOCUS       sequence-77212-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc22_4450
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             666..677
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttaatcacg ctgaaaacgt gcaatttctt ccagcatacg ttctgcacga
       61 ataacaccgg tgcctgcatg tgcacgatca atacgttcca gccacggatg ctgtgcacgc
      121 tgcagacgac gaaaacccag ccaggtgcca ataccaccgg caacaccaac cagcagattc
      181 agaaccagcc aatcggtcgg cagggtctga cctgtcagtg cacgccacag tgcatctgca
      241 actgcaatcc acagaaccca aaatgcaaca cccagccacg gtgcaacacg ggtacgccaa
      301 cgacgcagac gtgccagtgc atgctgggta cgcagcagcg gacctgcaac attaaactgg
      361 gtcagcagtt gcagctgaac cattgcgcta atggccagtg ccatacacca tgcctgcagc
      421 agaacaccac caataactgc aaccggctga tcggtatgcg gaatccagct acgtgcgcta
      481 acaattgcca gtgccagtgc acacagcagc tgtgctgtct gtgcaaccca cagcggacgc
      541 aggctgctac gaacggtttc ttcatgaaca acacccagca gctgaccggt ctgtgctgcc
      601 tgctgatcca gacgctgttc cagggtctgc catgcatgtt tcatatcgtc cagttccata
      661 tggtatttct cctctttctc tagtatttat tcgactataa caaaccattt tcttgcgtaa
      721 acctgtacga tcctacaggt cagccaacga tcgttccgat cagaacaaga aagctatgtg
      781 gcctgacgaa gcggcgcgcc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc
      841 gcccggaaga gagtcaattc agggtggtga atatgaaaaa cataaatgcc gacgacacat
      901 acagaataat taataaaatt aaagcttgta
//
