LOCUS       sequence-77216-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc25_1645
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             594..605
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttacgggct ttcggtggtt gccagtttcg gaatctgcag aaccggacga
       61 ttcagtgcaa caaccagtgc ttcttcaccc tgcggactgg tttcaaccag tgctgcggtc
      121 ggctgctgat ccggatcgtt ttttgcaaaa cgatcaaaat tcagacgagg catctgaccc
      181 ataacaccgg caacaaacag tgctgcaatt gcaccgctac cccacaggct cagggtacgc
      241 agctggctac gacgcagacg tgccagaaca cgctgaccca gatactgtgc gctaatgctg
      301 gtcggaatcg gcagcagctg cagtgcctgt ttcagctgac gcagctgctg atactgctgt
      361 ttaacctgcg gatcggtatc cagcagatgc tgaatttctt tacgttctgc cggtgttgct
      421 tcaccatcaa aataggcgct cagacgctga aactgttcaa aattatgacc catcggggtt
      481 tcgctatcgg tatgatgctg atggctggta tggttttttt tcatttccag atcaccacca
      541 cgacgacgat caccatcggt ctgacgctgg ctaaagcttt cgttcatatg gtatttctcc
      601 tctttctcta gtatttattc gactataaca aaccattttc ttgcgtaaac ctgtacgatc
      661 ctacaggtca gccaacgatc gttccgatca gaacaagaaa gctatgtggc ctgacgaagc
      721 ggcgcgccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
      781 gtcaattcag ggtggtgaat atgaaaaaca taaatgccga cgacacatac agaataatta
      841 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      901 aaatgg
//
