LOCUS       sequence-77217-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc27_1331
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             783..794
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttatgcacg cggaacaacg gtgcttgcca gcagaccggt atcggctgcg
       61 ctacaatgcg gtgcaacggc aaaaactgcc agtttatccg gtgtatctgc cggaataacc
      121 agcagcactg ccggacgggc atcaatatca atcggctgtg cacccagaac cggtgtgctt
      181 gccggataac ccagaccgct cagacagctg gtacgacggc tcggatcacc cagcggacca
      241 ccaggcggac cataatccgg tgtatgatgc agcagatcca gaacctgcgg acggctcagc
      301 ggaataaccg gtgccggttt gctaacggta atgtgctgtg cggtggtcgg tgcactcggt
      361 gccggaggcg gtgcatcaac aacggcacca acaccaattg cggttgcaac tgcacacaga
      421 cctgctgcac cggcaatcat acgaaccgga tgaacatgcg gacgggctgc atgtgctgca
      481 cccggactgc tattcggacg tgcgctacgc agtgctgcgc taatgctatc aacaactgcc
      541 ggacgtgctg ccggacccca tgccggatct gcacccattg ctgcaacatc acgacgaaca
      601 cgattcagtg cacgcagaat ctgctgagcc tgcggatcgc tacgaacacg gctacgaata
      661 cgtgctgcgg ttgcatcatc cagcagacct gcctgcagat ctgccagcag ttcaacggtc
      721 agaggcggat ctgcatctgc gctatgttta tccggatctt tatctgctgc gctcatatgg
      781 tatttctcct ctttctctag tatttattcg actataacaa accattttct tgcgtaaacc
      841 tgtacgatcc tacaggtcag ccaacgatcg ttccgatcag aacaagaaag ctatgtggcc
      901 tgacgaagcg gcgcgccatc gaatggcgca aa
//
