LOCUS       sequence-77218-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc27_4265
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             867..878
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttagctacg tgcatagcta tgttccagca gaatttttgc tttaccaact
       61 gcaggctgat caacacatgc ggtttcaaca atatatgcgg taacctgggt atcattggtt
      121 gcatccggca gaacaaccag cagtgcttca cgacctttat aaacacccgg ttcggttgca
      181 ataactgcat catcacgacc aataccttta cgaatacatt ccggaacggt cggctgttta
      241 aaaacatggt tttctgcggt ttcaccacca ttttcgcttt ccataccaaa gctacgcggt
      301 gtacggctac cattttctgc gctaccatct gcaaccagat tggtaacacg atctttcaga
      361 cgaccttcgc taaaggtatc tgcaattgcg gtctgctgtt cacgtgcggt attaccgctg
      421 ctgctatctt cggtcagact gctaacaaca acgctaccaa taccaatggc tgctgctgct
      481 gcaactgcac ccagaactgc aatacggcga cgaccaccac gacgacgatc tttacgaccc
      541 ggacctgtgc tgctcggacg tgcatgacct gccggacgat ctgccggtgt gctggtttca
      601 cggctaacac gggtgccatc tgcaataccc agaggcggtt ctgctgccag tgctgcatca
      661 atacgtgctg caacatcatc cggcatcggt gccggtgcag gcagggtgcc cagcagaccc
      721 tgaatttcgg tcaggcttgc ataaacatct gcacacagtt cacagctttc cagatgctga
      781 cgaacttcgg tggtacgggt ggtcggcagc agaccttctg ccagatctgc aatttctgca
      841 acatccggat gacctgtcat atggtatttc tcctctttct ctagtattta ttcgactata
      901 acaaaccatt ttcttgcgta aacctgtacg atcctacagg tcagccaacg atcgttcc
//
