LOCUS       sequence-77219-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc30_35
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             876..887
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttaacgaat ataacgttca ccaaccagca gattgctaat aacaccggtc
       61 ggaatggtaa actcggtaac acccatataa cccggtgcaa cttcatattc atcgaagctg
      121 atcaccagtt tatgatcttc ggtgatataa aaggtctgat ccggcagaat ggttttaaac
      181 ggttcggcat cttcatcggt cagccaatag attttattcg gatcttcttt catctgctgt
      241 ttcatttgtt ctttgatgtt ctggctgatc actttgatgt agcgttcatc tttaaacagg
      301 cttttcaggg tcagcaggat atcgtttttt ttgtcgatgg taatataacg gctctgggta
      361 tagctgcttg cctgggtggt ttcaatatta cgacgaatgc tcagcagatc cggtgtatcg
      421 gtaacggttt cataatcgct atagatgctc agatggcctt ttttgttttt gctggtgctc
      481 tgaataaact ctttatacag ctgctggctt tctttcagat atttttcatt aatgctattt
      541 tccagttctt tattgctcag accgctcagt gccggtgttt tcacatcaat gctgctctga
      601 tctttttctt ctttaatttc aataaaggtg atggctttca caattttgcc aataaccgga
      661 attttgctca ttgcctgtgc tgcatccgga ttaatgttaa ccagtgcggt aaacagaatt
      721 gctgctgcaa ttgctgcgct ggtcggccac ataacaatac gttttttttt cggttcttgc
      781 tgcagtgctt tttccacaat aatatccagt tctttcggaa tctgaacatt tttgtattct
      841 tcacgcagct gctgcagacg tttatccata tggtatttct cctctttctc tagtatttat
      901 tcgactataa caaaccattt tcttgcgtaa acctgtac
//
