LOCUS       sequence-77221-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc33_375
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             714..725
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttacggtgc tgcaactgcc agattaacaa atttttcacg ctgcatacga
       61 cccagcaggc ttgccagttc atcctggctc attgctttat caaactgcag gcgaaacata
      121 ccaccttttg catcaccaat aatgcttgcc tgatagctat ccagcagtgc ggtaatatct
      181 gcaacacgtg cttccggtgt aaaacgaacc agtgcacgtg ccggtgcaac tgctgcaccc
      241 agttcacggg taatcggtgc gctggtgctc aggcttgcgg tctgaaaggt ggtcggctgg
      301 gttttcatca gaactgcacc aataatacct gcctgcagaa ccagtgcaac ggcacccagg
      361 ctggctgacc atgccagggt acgcggactc aggcttgcaa aaaaggttgc aatacgtgcg
      421 ctcagcggca ggctaccggt ttcacgtgcc ggttctgcat caattgcacc aaacagtttc
      481 tgcattgcac gtgcgctcgg tgcacccagg ctttcattca gatgaatggt ttcttcatat
      541 tcaccacgaa ttgctgcata ctgttttgcc agttccggat cacgtgccag tgcatcttca
      601 acacgacgtg catcacgtgc attcagggtg cctgcagcat gccacggcag cagcagttca
      661 atttcgctcg gttcttgttc cagcattttt ttgctcaggg ccatcatatg gtatttctcc
      721 tctttctcta gtatttattc gactataaca aaccattttc ttgcgtaaac ctgtacgatc
      781 ctacaggtca gccaacgatc gttccgatca gaacaagaaa gctatgtggc ctgacgaagc
      841 ggcgcgccat cgaatggcgc aaa
//
