LOCUS       sequence-77223-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc33_423
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             852..863
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttacggtgc gctcagaaca acgctaacaa ccggttcttt ctgcagacga
       61 ccaatcaggg tttcttgatc ctgtttgctc aggctctggg tgccaaactg cagacgaaac
      121 agaccaccac ggctgctatc aacaacaact gcattatact gatccagcag tgctgcaatt
      181 tcgcttgcac gtgcttccgg tgcaaattta accagaacac gcggaccgct ctgcggtgcc
      241 aggctacgaa caaccggacc accgctacgt tctgccataa ccatcggagg ggtgctttta
      301 cctgcatctg ctgcacgatc tgccattgct gctgccggag ggctaacggt tgccggtgcc
      361 ggactacttg ccggtgcacg ggcaacttca cgctgcggct gatatgctgc ggtctgaacc
      421 ggtgctgcat gaggccaggt cagcagtgca ccaatcagac ctgcctgcag cagcagtgcc
      481 agacctgcaa cgcttgctga ccatgccagt gctttcgggc tcagacctgc aaaaaaacct
      541 gcaaaacgtg cacccagacc ctgacctgca cccggtgcac gtgccggttc tgcatcaatt
      601 gctgcaaaca gtttctgcat tgcacggctg ctcggtgcac ccaggctttc attcagttca
      661 atggtgcttg catattcttc cagaataact gcatactgac gtgccagtgc cggatcacga
      721 tccagtgcat ctgcaacacg acgtgcatca cgtgcattca gggtgcctgc tgcataccac
      781 ggcagcagtg cttcaacatc acccggttca cgcggatcag gcatggtatt gctcattgcc
      841 atcatatggt atttctcctc tttctctagt atttattcga ctataacaaa ccattttctt
      901 gcgtaaacct gtacgatcct acaggtcagc caacgatcgt tccgatca
//
