LOCUS       sequence-77224-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc35_3582
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             783..794
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttacggacg tttacgtgcg cttgctgcaa tggttgccag gcgttccaga
       61 cgttcaacca gattttcacc cggaaaacgt ttctgcagtt ctgccagctg ggttgctgcc
      121 tgttcggttt taccctgttc acgcagtgcc agaactgcac ccagtgcttc atccagaact
      181 tccggtttaa ctgcaactgc tttaccacgt gcctgtgcaa caaaatcgcc tgccagggct
      241 gcctgaactt ttgcctgctg tgctgcctgt gctggaggac gatctgccag tgctgccggt
      301 gccggaggtg ctgcttctgc catcagctgc ggtgcttcac cgctgcttgc atagcttgcc
      361 agcagtgcag gcggttcgct catttcaccg ctcagcggtg ccggtgctgc cggagcacgc
      421 attgcacgtt ttgctgctgc tgcaccggca acaccatcac gcagcggaac atcactaaat
      481 gcaacttccg gacgtgcctg acgttctgca tcatccagaa cattatggct aaccagaccc
      541 agaaccagtg caacacccag caggcttgca aatgctgcct gccaacgcgg acgacggctt
      601 gcttccagcc aacgacgcca ccaactacgt gccggttcaa catgtgctgc ctgacgacgt
      661 gctgctgcca gaattgcctg atccagtgcc ggtgcaggct gctgcggtgc atgtgcacga
      721 aaatgtgcca gcatttgttc ttcggcaacg cttgcgctat gtttctgcgg gttcatatgg
      781 tatttctcct ctttctctag tatttattcg actataacaa accattttct tgcgtaaacc
      841 tgtacgatcc tacaggtcag ccaacgatcg ttccgatcag aacaagaaag ctatgtggcc
      901 tgacgaagcg gcgcgccatc gaatggcgca aa
//
