LOCUS       sequence-77225-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR 
            insertion, Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Neo DEST (w18-1)
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      38..173
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    213..334
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(224..243)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     278..297
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             406..586
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    661..785
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atacgactca ctatagggcg aattgggtac gtccctaggc ctccaaaaaa gcctcctcac
       61 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
      121 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
      181 gttaggggcg ggatagctag agccagacat gataagatac attgatgagt ttggacaaac
      241 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      301 atttgtaacc attataagct gcaataaaca agttcctctc actctctgat attcatttct
      361 ttgcaagtta taaatactga ataataagat gacatgaact actactgcta gagattttcc
      421 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
      481 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
      541 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa
      601 gcagatcttg tcttcgttgg gagtgagcta gtccagtgtg gtggaattct gcagatatca
      661 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
      721 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      781 ctatggcggc cgcatta
//
