LOCUS       sequence-77226-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc37_2513
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             441..452
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttaatgggt atggctcaga tatgcattaa aaccatgatc cagatatgcg
       61 gtcagcggca gattatcgct cagcattgct gcatcaatat ctgccagttc tgcggtacgc
      121 tgcatatctt cccaatatgc aataccaatc agacctgcca gcagcagtgc cagaggccat
      181 gcacgtgcca gacgacgtgc caggctttta cgcggttcac caggcggacg cagttccagg
      241 gtgcctgcgc tacctgccag tgccggaacc agaacaattg ctgcatccgg ttttttacgt
      301 gccagtgctg cacgacgtgc tgctgccaga cgatcggtgg ttgcatccgg caggcttgct
      361 gcacgttcat ccagtgcacg acgaactttc agtgcaaaat caacttcttc tttggttgcc
      421 ggtgcgctgc tcatatggta tttctcctct ttctctagta tttattcgac tataacaaac
      481 cattttcttg cgtaaacctg tacgatccta caggtcagcc aacgatcgtt ccgatcagaa
      541 caagaaagct atgtggcctg acgaagcggc gcgccatcga atggcgcaaa acctttcgcg
      601 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatatg aaaaacataa
      661 atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc aataatgata
      721 ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat ttactcgcga
      781 tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat taccctaaaa
      841 aatggaggca atattatgat gacgctaatt taataaaata tgatcctata gtagattatt
      901 ctaactccaa tcattcac
//
