LOCUS       sequence-77262-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRa27_1331
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..209
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             831..848
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(901..919)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
      181 gcggcctgga agttctgttc caggggcccc atatgcctcc gcctattggt tattgtccgg
      241 cagttggttt tggtggtcgt catgaacgta gtgatgcaga actgctggca gcacatgttg
      301 ccggtgatcg ttatgcattt gatcagctgt ttcgtcgtca tcatcgtcag ctgcatcgtt
      361 tagcacgtct gaccagccgt accagcgaag atgcagatga tgcactgcag gatgcaatgc
      421 tgagcgcaca tcgtggtgca ggtagctttc gttatgatgc agcagttagc agctggctgc
      481 accgtattgt tgttaatgca tgtctggatc gtctgcgtcg tgcaaaagca catccgaccg
      541 caccgctgga agatgtttat ccggttgcag atcgtaccgc acaggttgaa accgcaattg
      601 cagttcagcg tgcactgatg cgtctgccgg ttgaacagcg tgcagccgtt gttgcagttg
      661 atatgcaggg ttatagcatt gcagatacca gtcgtatgct gggtgttgca gaaggcaccg
      721 ttaaaagccg ttgtgcacgt gcccgtgcgc gtctggctcg cctgctgggt tatctgaata
      781 ccggtgttaa tattcgtcgc taaggatcca agcttgcggc cgcactcgag caccaccacc
      841 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      901 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg a
//
