LOCUS       sequence-77272-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRa32_1122
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..209
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             789..806
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(859..877)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
      181 gcggcctgga agttctgttc caggggcccc atatgaccga aattcatctg cagaccaccg
      241 aaagcaccag cgttaatgat ggtctgccgc tgaatattga ttgggaaggt atttttcgtg
      301 aacatggtcg tcgtgtgcat cattttattc gtaaacgtgt tagccatcgt gaagatgccg
      361 aagatctgga acaaatgacc tggctggaag ttctgcgtaa tcgtgataaa tttgccggtg
      421 caagccgtcc ggaaacctgg gtttttggta ttgcactgaa tctggtgcgt aatcattttc
      481 gtctgcagag cggtcgtccg cgttgtgatg aactggaaga tgatattatt ctgacccagg
      541 gtgatgatcc gagccatatt accgaatatc agcgtctgct gaatagcacc ctgaatagta
      601 ttgcaaatct gccggaagat acccgtcgcc tgctgaacat gctggttgaa aaagatggta
      661 gctatcaggc aattgcagcc catctgaata ttccgattgg caccgttcgt agccgtctga
      721 gccgtgcacg tgttaccctg aaacagagcg tttttagcta aggatccaag cttgcggccg
      781 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      841 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      901 gggtcttg
//
