LOCUS       sequence-77276-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRa34_1384
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..209
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             849..866
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
      181 gcggcctgga agttctgttc caggggcccc atatgaatac ccgtacccgt ccgaccacca
      241 caccggttcc gcctgttgaa gaaacccgtt atgaagaaga actggcacac ggtctggtta
      301 aagcagatga agatgcattt gcagccattt atcgtcgttg gggtagcctg gttcataccc
      361 tggcaacccg tagcctgggt gatgcacatg aagcagaaga tgttacccag caggtttttg
      421 ttggtgcatg gcgtggtcgt catggttttc gtccggaacg tggcaccctg ggagcgtggc
      481 tggttggtat tacccgtcgt aaagttgttg atgcactggc agcacgcacc cgtcgtctga
      541 gcctggttga aagcgcaggt caggatatta caccggcacg tctggttcag cctgcactgg
      601 atgaagttct ggatcgtgtt ctgctggttg aagcactgag ccgtctgccg caggcacagc
      661 gtgatgttct gtgtatggca ttttatgaag atctgaccca ggcacagatt gcagaacgta
      721 ccggtgttcc gctgggcacc gttaaaagcc atgcacgtcg tggtctgcat cgtctgcgta
      781 ccgcagttgg tccgggtgca gcacatgata cctgtgttta aggatccaag cttgcggccg
      841 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      901 ctgagttggc tgctgcc
//
