LOCUS       sequence-77289-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRa41_491
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..209
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
      181 gcggcctgga agttctgttc caggggcccc atatgaccgt tgcagaaacc atttttgaaa
      241 cccgtcgtcg tgcaatgacc ggtctggcat atcgtatgct gggtagccgt gccgaagccg
      301 aagatgttgt tcaggatgca tggctgcgtt ggcgtggtgt tgatgttagc gaagttcaag
      361 aaccggcagc atttctgagc cgtgttgtta cccgtctggc actggatcgt ctgaaaagcg
      421 cacgtgcccg tcgtgaagtt tatgttggtg aatggctgcc ggaaccggtt gttgatccgg
      481 attttgatgc aggtcgtgat gcagatctga gcgttgcatt tctgctggcc ctggaacgcc
      541 tgacaccgct ggaacgtgca gcctttctgc tgcatgatgt ttttgatacc ccgtttgcag
      601 aaattgcagc aaccctgggt cgtagcgaaa ccgcatgtcg tcgtctggca agccgtgcac
      661 gtgaacatgt gcgtcgtgat cgtccgcgtc atcagccgag cgttgatcaa gaacgtcgtc
      721 tgaccgcagc attttttgaa gcagttatga ccggtgatga agcagcactg cgtgatctgc
      781 tggcacagga tgttgttatg catgcagatg gtggtggtct ggccagcgca aatctgaatc
      841 cggttcgtgg cctggcaaaa gcagttgcac tgattctggg tgttcgtcgt aaatggcctc
      901 cgcctagcgc aaccgttgtt cgcctggcac gtgttaatgg tcagcctggt ttgt
//
