LOCUS       sequence-77298-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination Vector for TALEColor-mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-NG(T)-mVenus
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(3..710,711..713,714..716))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(42..63)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     391..410
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     650..671
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
       61 atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actggtagct caggtagtgg
      121 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      181 agctgcacgc cgccgtcctc gatgttgtgg cggatcttga agttggcctt gatgccgttc
      241 ttctgcttgt cggcggtgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
      301 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      361 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      421 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      481 atgtggtcgg ggtagcgggc gaagcactgc aggccgtagc ccagggtggt cacgagggtg
      541 ggccagggca cgggcagctt gccggtggtg cagatcagct tcagggtcag cttgccgtag
      601 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      661 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatactt
      721 ccacttccct gcaggcctgc cactcgatgt gatgtcctct cgggaatcct tctgttggtc
      781 cgcttaatca atgcaggcgc gtgcggg
//
