LOCUS       sequence-77304-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses HA-Rab35S22N in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Scidmore Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDNA-HA-Rab35S22N
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             127..207
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(851..868)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    857..968
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 accatgtacc catacgatgt tccagattac gcttacccat acgatgttcc agattacgct
      181 tacccatacg atgttccaga ttacgctgat gcccgggact acgaccacct cttcaagctg
      241 ctcatcatcg gcgacagcgg tgtgggcaag aacagtttac tgttgcgttt tgcagacaac
      301 actttctcag gcagctacat caccacgatc ggagtggatt tcaagatccg gaccgtggag
      361 atcaacgggg agaaggtgaa gctgcagatc tgggacacag cggggcagga gcgcttccgc
      421 accatcacct ccacgtatta tcgggggacc cacggggtca ttgtggttta cgacgtcacc
      481 agtgccgagt cctttgtcaa cgtcaagcgg tggcttcacg aaatcaacca gaactgtgat
      541 gatgtgtgcc gaatattagt gggtaataag aatgacgacc ctgagcggaa ggtggtggag
      601 acggaagatg cctacaaatt cgccgggcag atgggcatcc agttgttcga gaccagcgcc
      661 aaggagaatg tcaacgtgga agagatgttc aactgcatca cggagctggt cctccgagca
      721 aagaaagaca acctggcaaa acagcagcag caacaacaga acgatgtggt gaagctcacg
      781 aagaacagta aacgaaagaa acgctgctgc taactcgagt ctagagggcc cgtttaaacc
      841 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      901 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      961 attgcatcgc attgtctgag taggtgtcat tcta
//
