LOCUS       sequence-7733-ex        7912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Sau S, Sun J, Lee CY
  TITLE     Molecular characterization and transcriptional analysis of type 8 
            capsule genes in Staphylococcus aureus.
  JOURNAL   J Bacteriol. 1997 Mar . 179(5):1614-21.
  PUBMED    9045821
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 1997 Mar . 179(5):1614-21."
COMMENT     Sequence Label: pCL52.2
FEATURES             Location/Qualifiers
     source          1..7912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(4168..4959)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(6211..7161)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      7209..7431
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttttt
       61 tattacctac aacttcttta gcacttaaac gaccatcatc agttattgga acaacgccat
      121 aatgcatatg tggtgttttt tcgtccatgt gaactgttgc atataataaa ttatctttac
      181 cgtattcttg ttctaaaaac tctttagcat attcaaaaaa ctgctttgta tcttctggcg
      241 tttgattatc aaagaaatca ttgtctgatg taattaaacc atcaatgtgt ttaatcgcgt
      301 ctgttctaat ttttcttttg cctgtataat tctgttcgat tttttcatca atcaagttat
      361 taaaattctg tttattagca tttaccaaat cataatttaa gtaagtttta ctatggtcta
      421 tatcttcatt ttcataatta ttattttctc tttgaacatg tttttgtatg cccgttgtat
      481 ttgttccaga tttaactttt gaaactctaa caatggaata agacatataa aaatcactcc
      541 taagcacaaa ggttttatgt gtatttaata gtttaagtct aacacactag acttattttt
      601 aataagtcgt taatacgtgt gctctgcgag gcttaaacct gtttttgcta acgcaaaaaa
      661 tgagtggcaa aatgctagcc actcatagtt ctaaaccaaa atataatata actattcaaa
      721 ctgcttttca gaacgtttta aatacaataa tcgtcaaaag acaacttaaa taataaggtt
      781 agccatggct acagaatatt actatacact ccagaagaat aatttataaa ttccagaact
      841 agtttacgat taatcaattg tagtgacaat aaacctccta caattgctat acctgttccc
      901 tctgataaaa aacttgtgaa atttagcaaa ctcattccag aagcaacttc ttcttcagaa
      961 agactacttg atactatttt tgatataact gttttagtaa aagataattc gcccataaca
     1021 aatataaaca taaaagtagt caaccacata ctaaactcaa caaaaaatgc aatagttaaa
     1081 aaacttatag agatagacaa tgatcctaaa ataaaaacaa ataatgatcc ttttctatcc
     1141 actaaaaaac caccaaaata accaaaaaca ataacactca tggttccagg aaaaataaca
     1201 ctattaccta ttgtcgctac atttacatga taaatagttt tcatcatata aggcaccatt
     1261 gatataaaac cagctactat agaaaatatt agcccaccag aaaacaaacc aagcataaac
     1321 ggaatgtttt tccctagttt aggattaata aaagggttag aaactcttga aatatgttta
     1381 ataaaaatca caaaaaagat tgtgaagagt attaaaaaag tccaattata atttgtcgta
     1441 aataacataa aacatataat acttatagac attaaaacaa tacctacgat atctaatgta
     1501 ttttttgttg atttaccagg taccattact ttaataagaa aaggtatagt tactattgta
     1561 atcataggaa gtataagtag gtaagaccaa tgaatataat gtgctattat tccccctatt
     1621 gaaggaccta acccttcacc taaagctaca attgatccta taaaaccaaa ggctttgcct
     1681 tgtttttttc ttgtaatatt tctagctaca accaccataa tcagtgaagg gaatgcagca
     1741 gatcctactc cttgtactaa cctaccaaaa atcaaaataa aaaagtgatt gtgaccaata
     1801 aaagcaatca atgaaccaag acagctcaaa ctaataccaa taattaacaa tttttttata
     1861 tttatataat cagataattt tccatatact gctgttccta tcgaaaaagt taacatatat
     1921 gcagtgttta cccagtttgt aattccagga gtagtattaa aatgatttgc aatatcaggt
     1981 aaagaaacat ttaaaaccat ttcatttaat acactaaaaa atgaaagaat acaaagccaa
     2041 aataaaacgc tataaaacaa acctttaaat tttttatata aactaaacaa ttttacctct
     2101 ttacttgagg tgactaaagt ttataggggt aaattattct ttcacctcat ataaattccc
     2161 caaattttta aatagacact tcataaaaaa atcctcctaa attagaatag tttaaatata
     2221 tcatttttgt tcagtaatat taatatgtac actatttcca aaatttaaat tcatgttgcc
     2281 aaaaatcgat tatgtctttt gcgcagtcgg cttaaaccag ttttcgctgg tgcgaaaaaa
     2341 gagtgtcttg tgacacctaa attcaaaatc tatcggtcag atttataccg atttgatttt
     2401 atatattctt gaataacata cgccgagtta tcacataaaa gcgggaacca atcatcaaat
     2461 ttaaacttca ttgcataatc cattaaactc ttaaattcta cgattccttg ttcatcaata
     2521 aactcaatca tttctttaat taatttatat ctatctgttg ttgttttctt taataattca
     2581 tcaacatcta caccgccata aactatcata tcttcttttt gatatttaaa tttattagga
     2641 tcgtccatgt gaagcatata tctcacaaga cctttcacac ttcctgcaat ctgcggaata
     2701 gtcgcattca attcttctgt taattatttt tatctgttca taagatttat taccctcata
     2761 catcactaga atatgataat gctctttttt catcctacct tctgtatcag tatccctatc
     2821 atgtaatgga gacactacaa attgaatgtg taactctttt aaatactcta accactcggc
     2881 ttttgctgat tctggatata aaacaaatgt ccaattacgt cctcttgaat ttttcttgtt
     2941 ttcagtttct tttattacat tttcgctcat gatataataa cggtgctaat acacttaaca
     3001 aaatttagtc atagataggc agcatgccag tgctgtctat ctttttttgt ttaaaatgca
     3061 ccgtattcct cctttgcata tttttttatt agaataccgg ttgcatctga tttgctaata
     3121 ttatattttt ctttgattct atttaatatc tcattttctt ctgttgtaag tcttaaagta
     3181 acagcaactt ttttctcttc ttttctatct acaactatca ctgtacctcc caacatctgt
     3241 ttttttcact ttaacataaa aaacaacctt ttaacattaa aaacccaata tttatttatt
     3301 tgtttggaca atggacaatg gacacctagg ggggaggtcg tagtaccccc ctatgttttc
     3361 tcccctaaat aaccccaaaa atctaagaaa aaaagacctc aaaaaggtct ttaattaaca
     3421 tctcaaattt cgcatttatt ccaatttcct ttttgcgtgt gatgcgctgc gtccattaaa
     3481 aatcctagag ctttgcaacc gaaagttaat agctgtcgct actactttcg cttacgctct
     3541 aagtatattt taaggactgt cacacgcaaa aagttttctc ggcataaaag tacctctaca
     3601 tctctaaatc gtctgtacgc tgtttctcac gctttctatc gatttgtttt tgcaattgtt
     3661 tttcgttcgc ttttaaagtc gatttcatta agtccgttaa atcgactggc gacagatccc
     3721 ctggctgaca ttttcggtgc gagtatccgt accattcaga actggcagga acagggaatg
     3781 cccgttctgc gaggcggtgg caagggtaat gaggtgcttt atgactctgc cgccgtcata
     3841 aaatggtatg ccgaaaggga tgctgaaatt gagaacgaaa agctgcgccg ggaggttgaa
     3901 gaactgcggc aggccagcga ggcagatctg gatctggatt ttaatgcgga tgttgcgatt
     3961 acttcgccaa ctattgcgat aacaagaaaa agccagcctt tcatgatata tctcccaatt
     4021 tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc tgatagtttg
     4081 gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg ccgcgaagcg
     4141 gcgtcggctt gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt
     4201 cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
     4261 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     4321 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     4381 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     4441 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     4501 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     4561 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     4621 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     4681 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     4741 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     4801 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
     4861 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     4921 tagttgagtc gatacttcgg cgatcaccgc ttccctcatg atgtttaact ttgttttagg
     4981 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
     5041 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aacagtcata
     5101 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
     5161 ccagttgcgt gagcgcatac gctacttgca ttacagctta cgaaccgaac aggcttatgt
     5221 ccactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag
     5281 cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac
     5341 gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct
     5401 gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac
     5461 cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca
     5521 gcttctgtat ggaacgggca tgcggatcag tgagggtttg caactgcggg tcaaggatct
     5581 ggatttcgat cacggcacga tcatcgtgcg ggagggcaag ggctccaagg atcgggcctt
     5641 gatgttaccc gagagcttgg cacccagcct gcgcgagcag gggaattaat tcccacgggt
     5701 tttgctgccc gcaaacgggc tgttctggtg ttgctagttt gttatcagaa tcgcagatcc
     5761 ggcttcagcc ggtttgccgg ctgaaagcgc tatttcttcc agaattgcca tgattttttc
     5821 cccacgggag gcgtcactgg ctcccgtgtt gtcggcagct ttgattcgat aagcagcatc
     5881 gcctgtttca ggctgtctat gtgtgactgt tgagctgtaa caagttgtct caggtgttca
     5941 atttcatgtt ctagttgctt tgttttactg gtttcacctg ttctattagg tgttacatgc
     6001 tgttcatctg ttacattgtc gatctgttca tggtgaacag ctttgaatgc accaaaaact
     6061 cgtaaaagct ctgatgtatc tatctttttt acaccgtttt catctgtgca tatggacagt
     6121 tttccctttg atatgtaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct
     6181 tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt
     6241 ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatactt
     6301 actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa
     6361 agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg taatggttgt
     6421 tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat
     6481 ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac
     6541 caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg
     6601 gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc
     6661 aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca
     6721 ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt
     6781 tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa
     6841 tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa
     6901 aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc
     6961 ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac
     7021 cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa
     7081 aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt
     7141 gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca
     7201 attgagatgg gctagtcaat gataattact agtccttttc ctttgagttg tgggtatctg
     7261 taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc
     7321 cggtagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa
     7381 gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta
     7441 gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag
     7501 accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat
     7561 tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg
     7621 ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga
     7681 ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt
     7741 ttatggcggg tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct
     7801 ctgattttcc agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat
     7861 gcacccagta aggcagcggt atcatcaaca ggcttacccg tcttactgtc gg
//
