LOCUS       sequence-77355-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Arf6Q67L-EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  TITLE     Scidmore Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Arf6Q67L-EGFP
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      638..647
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(689..710)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(950..969)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tcatggggaa ggtgctatcc aaaatcttcg ggaacaagga
      121 aatgcggatc ctcatgttgg gcctggacgc ggccggcaag acaacaatcc tgtacaagtt
      181 gaagctgggc cagtcggtga ccaccattcc cactgtgggt ttcaacgtgg agacggtgac
      241 ttacaaaaat gtcaagttca acgtatggga tgtgggcggc ctggacaaga tccggccgct
      301 ctggcggcat tactacactg ggacccaagg tctcatcttc gtagtggact gcgccgaccg
      361 cgaccgcatc gatgaggctc gccaggagct gcaccgcatt atcaatgacc gggagatgag
      421 ggacgccata atcctcatct tcgccaacaa gcaggacctg cccgatgcca tgaaacccca
      481 cgagatccag gagaaactgg gcctgacccg gattcgggac aggaactggt atgtgcagcc
      541 ctcctgtgcc acctcagggg acggactcta tgaggggctc acatggttaa cctctaacta
      601 caaatctgtc gacggtaccg cgggcccggg atccatcgcc accatggtga gcaagggcga
      661 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      721 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      781 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      841 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
      901 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      961 ctacaagacc cgcgccgagg tgaagttcga gggcgaca
//
