LOCUS       sequence-77386-e        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of AAVS1-targeting sgRNA; insert can be 
            replaced with custom sgRNA (see protocol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Wang T, Wei JJ, Sabatini DM, Lander ES
  TITLE     Genetic screens in human cells using the CRISPR-Cas9 system.
  JOURNAL   Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. 
            Epub 2013 Dec 12.
  PUBMED    24336569
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1246981"; journalName: "Science"; date: "2014-01-3-
            3"; volume: "343"; issue: "6166"; pages: "80-4"
COMMENT     Sequence Label: pLX-sgRNA
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..32
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14..32
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        60..286
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             287..467
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    514..639
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
       61 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
      121 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
      181 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
      241 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
      301 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      361 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      421 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
      481 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
      541 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
      601 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
      661 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
      721 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
      781 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
      841 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
      901 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
      961 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1021 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1081 caccaaggca a
//
