LOCUS       sequence-77387-e        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of AAVS1-targeting sgRNA; insert can be 
            replaced with custom sgRNA (see protocol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Wang T, Wei JJ, Sabatini DM, Lander ES
  TITLE     Genetic screens in human cells using the CRISPR-Cas9 system.
  JOURNAL   Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. 
            Epub 2013 Dec 12.
  PUBMED    24336569
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1246981"; journalName: "Science"; date: "2014-01-3-
            3"; volume: "343"; issue: "6166"; pages: "80-4"
COMMENT     Sequence Label: pLX-sgRNA
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..407)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(419..919)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(482..500)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 tgcagctgca ggccctccca cacataacca gagggcagca attcacgaat cccaactgcc
       61 gtcggctgtc catcactgtc cttcactatg gctttgatcc caggatgcag atcgagaagc
      121 acctgtcggc accgtccgca ggggctcaag atgcccctgt tctcatttcc gatcgcgacg
      181 atacaagtca ggttgccagc tgccgcagca gcagcagtgc ccagcaccac gagttctgca
      241 caaggtcccc cagtaaaatg atatacattg acaccagtga agatgcggcc gtcgctagag
      301 agagctgcgc tggcgacgct gtagtcttca gagatgggga tgctgttgat tgtagccgtt
      361 gctctttcaa tgagggtgga ttcttcttga gacaaaggct tggccatggt ggtaccccct
      421 ggggagagag gtcggtgatt cggtcaacga gggagccgac tgccgacgtg cgctccggag
      481 gcttgcagaa tgcggaacac cgcgcgggca ggaacagggc ccacactacc gccccacacc
      541 ccgcctcccg caccgcccct tcccggccgc tgctctcggc gcgccctgct gagcagccgc
      601 tattggccac agcccatcgc ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg
      661 cgagggtact agtgagacgt gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca
      721 aggaaccttc ccgacttagg ggcggagcag gaagcgtcgc cggggggccc acaagggtag
      781 cggcgaagat ccgggtgacg ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg
      841 cgccgctgcg tttcccggaa ccacgcccag agcagccgcg tccctgcgca aacccagggc
      901 tgccttggaa aaggcgcaag ggtcccctcg gggttgggag gtgggtctga aacgataatg
      961 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
     1021 ctaccaagcc tcctactatc attat
//
