LOCUS       sequence-77389-e        1137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen bicistronic lentiviral vector allows for simultaneous 
            expression of a transgene and EGFP marker to facilitate tracking of 
            transduced cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1137)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI
FEATURES             Location/Qualifiers
     source          1..1137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    200..233
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    377..965
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             483..494
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     892..912
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     978..994
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     979..995
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1116..1137)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
       61 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
      121 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
      181 agtacaagca aaaagcagca taacttcgta taatgtatgc tatacgaagt tattcccatg
      241 gtgaattgat cccatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt
      301 cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtaccg
      361 agctcgaatt cccgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
      421 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
      481 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
      541 caggcgagca gccatggaaa ggacgtcagc ttccccgaca acaccacgga attgtcagtg
      601 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
      661 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
      721 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
      781 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
      841 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
      901 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
      961 tgattatcgg aattccctcg aggtcgacgg tatcgcgata agcttgatat cgaattcgaa
     1021 gcttgagctc gactagctag ctagctacta gctagtcgag atctgagtcc ggacttgtac
     1081 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatg
//
