LOCUS       sequence-77401-e        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cumate-inducible expression of SYFP2-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQY
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..30
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    73..100
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             join(122..124,125..127,128..838)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(167..188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(428..447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     775..796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             890..955
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(1027..1044)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1027..1043)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1036..1058)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggaacaaaca gacaatctgg tctgtttgta catggttgac ggatcgccgc gaagccgcta
       61 actgcgccgg ttaacaaaca gacaatctgg tctgtttgta actagtagag gaagcttccg
      121 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      181 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      241 cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac
      301 cctcgtgacc accctgggct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa
      361 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      421 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      481 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      541 caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa
      601 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
      661 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      721 ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
      781 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagat
      841 taatatgact ctagaagatc tcgaggtacc gagctccaat tgggatccgg actacaagga
      901 ccacgacggc gactataagg accacgacat cgactacaag gatgacgacg acaagtgagg
      961 atccggtgaa gtgacccgca aaaaaaaagg ccaggggatc gctcccccgg cctttttttt
     1021 gaattcactg gccgtcgttt tacaacgtcg tgactggg
//
