LOCUS       sequence-77415-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  this report is the first to directly measure nAChr subunit 
            stoichiometry using FRET and plasma membrane localization of Alpha6 
            and Beta3 containing receptors using TIRF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Drenan RM, Nashmi R, Imoukhuede P, Just H, McKinney S, Lester HA
  TITLE     Subcellular trafficking, pentameric assembly, and subunit 
            stoichiometry of neuronal nicotinic acetylcholine receptors 
            containing fluorescently labeled alpha6 and beta3 subunits.
  JOURNAL   Mol Pharmacol. 2008 Jan;73(1):27-41. Epub 2007 Oct 11.
  PUBMED    17932221
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2008-01"; volume: "73(1)"; pages: "27-41. Epub 
            2007 Oct 11"
COMMENT     Sequence Label: pcDNA3.1-nAChr-Alpha6-GFP
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(306..557)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(348..369)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     697..716
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     956..977
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtttaaacgg gccctctaga ctcgagttaa gactttcctg tgttcccaag cagtggctgc
       61 agaaatagcc ccacagttcc aaacacacag acaattataa atacccaaag gaagactctg
      121 tccaccacca tagccatgta tttccagtcg tcttctacct cgtttgtttc attgtggctc
      181 ttcatgtttt ctgctatgaa ttgaacactc tcgatgacat cttcaacatc ggacgagtgc
      241 tctgaattct ctgccaccca ccgtgcaggc tgctggcttg atcttctctt tccaggtgct
      301 cctgccttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
      361 aggaccatgt gatcgcgctt ctcgttgggg tctttgctaa gcttggactg ggtgctcagg
      421 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
      481 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
      541 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
      601 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
      661 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
      721 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
      781 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
      841 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
      901 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
      961 ccgtccagct cgaccag
//
