LOCUS       sequence-77430-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  this report is the first to directly measure nAChr subunit 
            stoichiometry using FRET and plasma membrane localization of Alpha6 
            and Beta3 containing receptors using TIRF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Drenan RM, Nashmi R, Imoukhuede P, Just H, McKinney S, Lester HA
  TITLE     Subcellular trafficking, pentameric assembly, and subunit 
            stoichiometry of neuronal nicotinic acetylcholine receptors 
            containing fluorescently labeled alpha6 and beta3 subunits.
  JOURNAL   Mol Pharmacol. 2008 Jan;73(1):27-41. Epub 2007 Oct 11.
  PUBMED    17932221
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2008-01"; volume: "73(1)"; pages: "27-41. Epub 
            2007 Oct 11"
COMMENT     Sequence Label: pCDNA3.1-nAChr-beta3-YFP
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(447..698)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(489..510)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     838..857
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtttaaacgg gccctagtaa cggccgccag tgtgctggaa ttcgcccttg gctcccagga
       61 caaggcaagg gcaccacaaa tgttgcatgc aactcctttc tgtgaacaca ccagcagcac
      121 tcagttctag gtgggattct ctctaggtgt gtcttctaca tggatccaga gagcagttcc
      181 taatggaaac gatgtatcca catcttcaag gctggaataa aaatcaggac agagcccaaa
      241 accgaagctg tcagaaagag ccagaggaag atgcggtcaa gaacctgagc cacgaatttc
      301 cagtcttgaa caacctgact gatgaagtgt tccttcttca catgcctcga gatgtatctg
      361 atggactcag aggccttctc caggaaagcg accagaactc tttctccatc actggtagga
      421 gtctgcttct ttttccctgc tcctgccttg tacagctcgt ccatgccgag agtgatcccg
      481 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      541 agggcggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      601 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      661 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      721 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      781 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      841 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      901 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa gcactgcagg
      961 ccgtaaccga aggtggtcac gagggtgggc cagg
//
