LOCUS       sequence-77432-e        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  this report is the first to directly measure nAChr subunit 
            stoichiometry using FRET and plasma membrane localization of Alpha6 
            and Beta3 containing receptors using TIRF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Drenan RM, Nashmi R, Imoukhuede P, Just H, McKinney S, Lester HA
  TITLE     Subcellular trafficking, pentameric assembly, and subunit 
            stoichiometry of neuronal nicotinic acetylcholine receptors 
            containing fluorescently labeled alpha6 and beta3 subunits.
  JOURNAL   Mol Pharmacol. 2008 Jan;73(1):27-41. Epub 2007 Oct 11.
  PUBMED    17932221
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2008-01"; volume: "73(1)"; pages: "27-41. Epub 
            2007 Oct 11"
COMMENT     Sequence Label: pCDNA3.1-nAChr-beta3-CFP
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(492..513)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     841..860
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggtttaaacg ggccctctag taacggccgc cagtgtgctg gaattcgccc ttggctccca
       61 ggacaaggca agggcaccac aaatgttgca tgcaactcct ttctgtgaac acaccagcag
      121 cactcagttc taggtgggat tctctctagg tgtgtcttct acatggatcc agagagcagt
      181 tcctaatgga aacgatgtat ccacatcttc aaggctggaa taaaaatcag gacagagccc
      241 aaaaccgaag ctgtcagaaa gagccagagg aagatgcggt caagaacctg agccacgaat
      301 ttccagtctt gaacaacctg actgatgaag tgttccttct tcacatgcct cgagatgtat
      361 ctgatggact cagaggcctt ctccaggaaa gcgaccagaa ctctttctcc atcactggta
      421 ggagtctgct tctttttccc tgctcctgcc ttgtacagct cgtccatgcc gagagtgatc
      481 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      541 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      601 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
      661 cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat atagacgttg
      721 tggctgatgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      781 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      841 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
      901 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc
      961 acgccccagg tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
     1021 ca
//
