LOCUS       sequence-77439-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human SDHB in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Sancak Y, Markhard AL, Kitami T, Kovacs-Bogdan E, Kamer KJ, Udeshi 
            ND, Carr SA, Chaudhuri D, Clapham DE, Li AA, Calvo SE, Goldberger O,
            Mootha VK
  TITLE     EMRE is an Essential Component of the Mitochondrial Calcium 
            Uniporter Complex.
  JOURNAL   Science. 2013 Nov 14.
  PUBMED    24231807
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Nov 14."
COMMENT     Sequence Label: pLYS5-SDHB-Flag
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..25)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(930..968)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 acttgtcatc gtcatccttg taatccatag atccaccaga tccaccagat ccacccccgg
       61 gaactgaagc tttcttctcc ttataggttg ccatcatttt cttgatctct gcaatagctt
      121 tccctggatt cagaccctta ggacaggtcc ttgtgcagtt catgatggtg tggcagcggt
      181 atagagagaa tgggtcctgc agcttggcca ggcgctcctc tgtgaagtca tctctggagt
      241 caatcatcca gcgataggcc tgcataagaa ctgcaggccc cagatatttg tctccgttcc
      301 accagtagct ggggcagctg gtgctacagc aggcacagag aatgcactcg tagagcccgt
      361 ccagtttctc acgctcttct atggactgca gatactgctg cttgccttcc tgagattcat
      421 ccttcttctt caaataaggc tcaatggatt tgtactgtgc atagaagttg ctcaaatcgg
      481 gaacaagatc ctttatcaca tacatgtgtg gaagagggta gatttttgag accttattga
      541 ggttggtgtc aatccttcgg gtgcaagcta gagtgttgcc tccattgatg ttcattgcac
      601 aagagccaca gatgccttct ctgcatgatc ttcggaaggt caaagtagag tcaacttcat
      661 tcttaatctt gattaaagca tccaatacca tggggccaca tttattaagg tcaacttcat
      721 aagtctgcat atgaggtttg tctccagcct tgtctgggtc ccatcgatag atggcaaatt
      781 tcttgatacg gggagctgtg gctgcagctg tctgggctcc tcgggaggcc tgcaggcagg
      841 ctccgccaag ggttgtggcc ggcaaccggc gcctcaagga gagtgcgacc accgccgcca
      901 tgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca
      961 cgcctacc
//
