LOCUS       sequence-77444-e         510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression plasmid for N-terminal his tagged S.aureus SrtA delta 59.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Guimaraes CP, Witte MD, Theile CS, Bozkurt G, Kundrat L, Blom AE, 
            Ploegh HL
  TITLE     Site-specific C-terminal and internal loop labeling of proteins 
            using sortase-mediated reactions.
  JOURNAL   Nat Protoc. 2013 Sep;8(9):1787-99. doi: 10.1038/nprot.2013.101. Epub
            2013 Aug 29.
  PUBMED    23989673
REFERENCE   2  (bases 1 to 510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.101"; journalName: "Nat Protoc"; date: 
            "2013-09"; volume: "8"; issue: "9"; pages: "1787-99"
COMMENT     Sequence Label: pET28a-SrtAdelta59
FEATURES             Location/Qualifiers
     source          1..510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..60
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgagaggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc
       61 catatgcaag ctaaacctca aattccgaaa gataaatcga aagtggcagg ctatattgaa
      121 attccagatg ctgatattaa agaaccagta tatccaggac cagcaacacc tgaacaatta
      181 aatagaggtg taagctttgc agaagaaaat gaatcactag atgatcaaaa tatttcaatt
      241 gcaggacaca ctttcattga ccgtccgaac tatcaattta caaatcttaa agcagccaaa
      301 aaaggtagta tggtgtactt taaagttggt aatgaaacac gtaagtataa aatgacaagt
      361 ataagagatg ttaagcctac agatgtagga gttctagatg aacaaaaagg taaagataaa
      421 caattaacat taattacttg tgatgattac aatgaaaaga caggcgtttg ggaaaaacgt
      481 aaaatctttg tagctacaga agtcaaataa
//
