LOCUS       sequence-77451-e         564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression plasmid for N-terminal his tagged S.pyogenes SrtA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Guimaraes CP, Witte MD, Theile CS, Bozkurt G, Kundrat L, Blom AE, 
            Ploegh HL
  TITLE     Site-specific C-terminal and internal loop labeling of proteins 
            using sortase-mediated reactions.
  JOURNAL   Nat Protoc. 2013 Sep;8(9):1787-99. doi: 10.1038/nprot.2013.101. Epub
            2013 Aug 29.
  PUBMED    23989673
REFERENCE   2  (bases 1 to 564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.101"; journalName: "Nat Protoc"; date: 
            "2013-09"; volume: "8"; issue: "9"; pages: "1787-99"
COMMENT     Sequence Label: pET28a-SpySrtA
FEATURES             Location/Qualifiers
     source          1..564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgcgcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggatccgtc
       61 ttgcaagcac aaatggcggc tcagcaactt cctgttatag ggggcattgc cataccagag
      121 cttggcatta atttaccaat ttttaaaggt ttaggaaata ctgagcttat ttatggcgca
      181 ggaacgatga aagaagaaca agttatggga ggagaaaata attattctct tgccagtcat
      241 catatttttg gaattacagg ttcatctcaa atgctctttt cgccgcttga aagagcacaa
      301 aatgggatgt ccatctattt aacagataaa gaaaaaattt acgaatacat cataaaagat
      361 gttttcacgg tagctcctga acgcgttgat gttatcgatg atacagctgg tctcaaagaa
      421 gtgactttag tgacttgtac agatatcgaa gcaacagaac gtattattgt caaaggagaa
      481 ctaaaaacag aatacgactt tgataaagcg cccgccgatg tattgaaagc ttttaatcat
      541 tcttataacc aagtatctac ctag
//
