LOCUS       sequence-77453-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses GAL4BD-Rab14S25N-Delta-CGC in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  TITLE     Scidmore Lab GAL4BD plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GBK-Rab14S25N-Delta-CGC
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             52..81
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      768..815
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(897..921)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tcgccggaat ttgtaatacg actcactata gggcgagccg ccatcatgga ggagcagaag
       61 ctgatctcag aggaggacct gcatatggcc atggaggccg aattcatggc aactacaccg
      121 tacaactact cttacatctt taaatatatt attattgggg acatgggagt aggaaaaaat
      181 tgcttgcttc atcaatttac agaaaaaaaa tttatggctg attgtcctca cacaattggt
      241 gttgaatttg gtacaagaat aatcgaagtt agtggccaaa aaataaaact gcagatttgg
      301 gatacggcag gacaggagcg atttagggct gttacacgga gctactacag aggagctgcg
      361 ggagctctta tggtctatga tatcactaga agaagtacat ataaccactt aagcagctgg
      421 ttgacagatg caaggaatct caccaatcca aatactgtaa taattctcat aggaaataaa
      481 gcagatttgg aggcacagag agatgttaca tatgaagaag ccaaacagtt tgctgaagaa
      541 aatggcttat tgttcctcga agcgagtgca aaaacgggag agaatgtaga agatgccttc
      601 cttgaggctg ccaagaaaat ctatcagaac attcaggatg gaagcttgga tctgaatgct
      661 gctgagtctg gtgtacaaca caaaccttca gccccgcagg gaggccggct aaccagtgaa
      721 ccccaacccc agagagaagg ctagctcgac ctgcagcggc cgcataacta gcataacccc
      781 ttggggcctc taaacgggtc ttgaggggtt ttttgcgcgc ttgcagccaa gctaattccg
      841 ggcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
      901 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
      961 tttcctgtag gtcaggttgc tttctcaggt atagcatg
//
