LOCUS       sequence-77454-e         471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression plasmid for C-terminal his tagged S. aureus SrtA with 
            enhanced catalytic activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Shi J, Kundrat L, Pishesha N, Bilate A, Theile C, Maruyama T, Dougan
            SK, Ploegh HL, Lodish HF
  TITLE     Engineered red blood cells as carriers for systemic delivery of a 
            wide array of functional probes.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10131-6. doi: 
            10.1073/pnas.1409861111. Epub 2014 Jun 30.
  PUBMED    24982154
REFERENCE   2  (bases 1 to 471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1409861111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-07-15- 15"; volume: "111"; issue: "28"; pages: "10131-6"
COMMENT     Sequence Label: pet30b-5M SrtA
FEATURES             Location/Qualifiers
     source          1..471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             451..468
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaagcta aacctcaaat tccgaaagat aaatcaaaag tggcaggcta tattgaaatt
       61 ccagatgctg atattaaaga accagtatat ccaggaccag caacacgcga acaattaaat
      121 agaggtgtaa gctttgcaga agaaaatgaa tcactagatg atcaaaatat ttcaattgca
      181 ggacacactt tcattgaccg tccgaactat caatttacaa atcttaaagc agccaaaaaa
      241 ggtagtatgg tgtactttaa agttggtaat gaaacacgta agtataaaat gacaagtata
      301 agaaacgtta agccaacagc tgtagaagtt ctagatgaac aaaaaggtaa agataaacaa
      361 ttaacattaa ttacttgtga tgattacaat gaagagacag gcgtttggga aacacgtaaa
      421 atctttgtag ctacagaagt caaactcgag caccaccacc accaccactg a
//
