LOCUS       sequence-77456-e         471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression plasmid for C-terminal his tagged, calcium independent 
            S.aureus SrtA with enhanced catalytic activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 471)
  TITLE     unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pet30b-7M SrtA
FEATURES             Location/Qualifiers
     source          1..471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             451..468
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaagcta aacctcaaat tccgaaagat aaatcaaaag tggcaggcta tattgaaatt
       61 ccagatgctg atattaaaga accagtatat ccaggaccag caacacgcga acaattaaat
      121 agaggtgtaa gctttgcaaa agaaaatcaa tcactagatg atcaaaatat ttcaattgca
      181 ggacacactt tcattgaccg tccgaactat caatttacaa atcttaaagc agccaaaaaa
      241 ggtagtatgg tgtactttaa agttggtaat gaaacacgta agtataaaat gacaagtata
      301 agaaacgtta agccaacagc tgtagaagtt ctagatgaac aaaaaggtaa agataaacaa
      361 ttaacattaa ttacttgtga tgattacaat gaagagacag gcgtttggga aacacgtaaa
      421 atctttgtag ctacagaagt caaactcgag caccaccacc accaccactg a
//
