LOCUS       sequence-77458-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses GAL4BD-Rab30Q68LdeltaCCNFN in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  TITLE     Scidmore Lab GAL4BD plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GBK-Rab30Q68LdeltaCCNFN
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             52..81
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      732..779
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      806..993
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(861..885)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tcgccggaat ttgtaatacg actcactata gggcgagccg ccatcatgga ggagcagaag
       61 ctgatctcag aggaggacct gcatatggcc atggaggccg aattcccggg gatccatatg
      121 agtatggaag attatgattt cctgttcaaa attgttttaa ttggcaacgc tggtgtgggg
      181 aagacgtgcc tcgtccgaag attcactcag ggtcttttcc ccccaggtca aggagccaca
      241 attggagttg attttatgat taagacagtg gagattaatg gtgaaaaagt aaagctacag
      301 atctgggaca cagcaggtct agagagattt cggtccatta cccagagtta ctaccgaagc
      361 gccaatgcct tgatcctcac ctatgacatt acctgtgagg aatccttccg ttgccttcct
      421 gagtggctgc gggagataga acaatatgcc agcaacaagg tcatcactgt gttagtgggc
      481 aacaagattg acctggctga aaggagagag gtttcccagc agcgagctga agaattctca
      541 gaagctcagg acatgtatta tctggagacc tcagccaagg aatctgataa tgtggagaaa
      601 ctcttccttg acttagcatg ccgactcatc agtgaggcca gacagaacac acttgtgaac
      661 aatgtatcct cacccttacc tggagaaggg aaaagcatca gctatttgac ttaactgcag
      721 cggccgcata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      781 gcgcttgcag ccaagctaat tccgggcgaa tttcttatga tttatgattt ttattattaa
      841 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
      901 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca
      961 tgaggtcgct cttattgacc acacctctac cggcatg
//
