LOCUS       sequence-77460-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:Venus-Tub1+3'UTR::HPH
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..775)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 atctagcctg gacgggcaaa tcgaacatac cattaatact aataacttct ctcatggatc
       61 ctttgtacaa ttcatccata ccatgggtaa taccagcagc agtaacaaat tctaacaaga
      121 ccatgtggtc tctcttttcg tttggatctt tggataaggc agattgatag gataagtaat
      181 ggttgtctgg taacaagact ggaccatcac caattggagt attttgttga taatggtcag
      241 ctaattgaac accaccatct tcaatgttgt gtctaatttt gaagttagct ttgataccat
      301 tcttttgttt gtcagcagtg atgtaaacat tgtgagagtt atagttgtat tccaatttgt
      361 gacctaaaat gttaccatct tctttaaaat caataccttt taattcgatt ctattaacta
      421 aggtatcacc ttcaaacttg acttcagctc tggtcttgta gttaccgtca tctttgaaaa
      481 aaatagttct ttcttgaaca taaccttctg gcatggcaga cttgaaaaag tcatgttgtt
      541 tcatatgatc tgggtatcta gcaaaacatt gcaaaccata acctaaagta gtgactaagg
      601 ttggccatgg aactggcaat ttaccagtag tacaaatcaa ttttaaggtc aatttaccgt
      661 aagtagcatc accttcacct tcaccggaga cagaaaattt gtgaccatta acatcaccat
      721 ctaattcaac caaaattggg acaacaccag tgaataattc ttcaccttta gacatgcttt
      781 gccttcgttt atcttgcctg ctcatttttt agtatattct tcgaagaaat cacattactt
      841 tatataatg
//
