LOCUS       sequence-77461-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mEos2-Tub1+3'UTR::HPH
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..734)
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gcctggacgg gcaaatcgaa cataccatta atactaataa cttctctcat ggatcctcgt
       61 ctggcattgt caggcaatcc agaatgagca acagcatgct catacagctt aaccttgttg
      121 taatctttgt catggcttaa aatctcaatg cagtggtcca caaagtggta gcctggtaac
      181 ttgacaccct tctccttagc tttgtaagta gttctgaagt cacatcggta atgggcattt
      241 ccttcaagca acaaagccat atgaatatca cccgtcagca ctccatcacg cacatacatt
      301 ttctcagtgg agggctccca tttcagcgtc ttcttctgca taactggacc attggcggga
      361 aagttggtac cataaaatcg aactttatta tagaaagtgt ccccttccat tgttatgtcg
      421 tttctggcaa tgcaaatgcc cccgtcttcg aaagtcaagc ttcgttccca cgaatacccc
      481 ttaggaaacg actgcttaaa atagtcttgt atgttgtctg gatatttggc gaataccctg
      541 ttgccgtaat ggaatgcagt ggtcaggata tcaaaggcaa aaggcagagg tccgccctct
      601 ttgacttcaa gatccatact ctgttttccc tcaaaaggct tgcctgtacc atctccgtcg
      661 atcacaaagt ggtgcccgtt tacgttgcct tccatacgga gtttgatctt catgtctggc
      721 ttaatcgcac tcatctcgag ctttgccttc gtttatcttg cctgctcatt ttttagtata
      781 ttcttcgaag aaatcacatt actttatata atgtataatt cattatgtga taatgccaat
      841 cgctaa
//
