LOCUS       sequence-77466-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:CloverGFP-Tub1+3'UTR::URA3
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(16..726,727..729,730..732))
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(58..79)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     666..687
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttctctcatg gatcccttgt acagctcgtc catgccatgt gtaatcccgg cggcggtcac
       61 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      121 atggctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      181 ctggtagtgg tcggcgagct gcacgctgcc gtcctcaacg ttgtggcgga tcttgaagtt
      241 agccttgatg ccgttcttct gcttgtcggc cgtgatatag acgttgtggc tgttgaagtt
      301 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      361 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtaggtacc
      421 gtcgtccttg aaagagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
      481 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag caggccacgc cgtagccgaa
      541 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
      601 ggtcagcttg ccgttggtgg catcgccctc gccctcgccg cggacgctga acttgtggcc
      661 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
      721 cttgctcacc atctcgagct ttgccttcgt ttatcttgcc tgctcatttt ttagtatatt
      781 cttcgaagaa atcacattac tttatataat g
//
