LOCUS       sequence-77468-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:Venus-Tub1+3'UTR::URA3
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..729)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ttctctcatg gatcctttgt acaattcatc cataccatgg gtaataccag cagcagtaac
       61 aaattctaac aagaccatgt ggtctctctt ttcgtttgga tctttggata aggcagattg
      121 ataggataag taatggttgt ctggtaacaa gactggacca tcaccaattg gagtattttg
      181 ttgataatgg tcagctaatt gaacaccacc atcttcaatg ttgtgtctaa ttttgaagtt
      241 agctttgata ccattctttt gtttgtcagc agtgatgtaa acattgtgag agttatagtt
      301 gtattccaat ttgtgaccta aaatgttacc atcttcttta aaatcaatac cttttaattc
      361 gattctatta actaaggtat caccttcaaa cttgacttca gctctggtct tgtagttacc
      421 gtcatctttg aaaaaaatag ttctttcttg aacataacct tctggcatgg cagacttgaa
      481 aaagtcatgt tgtttcatat gatctgggta tctagcaaaa cattgcaaac cataacctaa
      541 agtagtgact aaggttggcc atggaactgg caatttacca gtagtacaaa tcaattttaa
      601 ggtcaattta ccgtaagtag catcaccttc accttcaccg gagacagaaa atttgtgacc
      661 attaacatca ccatctaatt caaccaaaat tgggacaaca ccagtgaata attcttcacc
      721 tttagacatg ctttgccttc gtttatcttg cctgctcatt ttttagtata ttcttcgaag
      781 aaatcacatt actttatata a
//
