LOCUS       sequence-77469-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mRuby2-Tub1+3'UTR::URA3
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..767)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gcctggacgg gcaaatcgaa cataccatta atactaataa cttctctcat ggatcccttg
       61 tacagctcgt ccatcccacc accaagcccg gcgaacttgg caactgcgtg ttcgcgttgt
      121 actacgaaca tttcattgtc actttcctct aacctttcca ggcggtgatc aacggcatgg
      181 ataccgggca tcttgatgtt cccgacggtc ttttttgacc tgtaagttgt tacgaaagag
      241 caagacagat ggccaccacc atcaactttc agtgccatat gagtgtatcc cctcagacca
      301 ccatctgctg gatacatcat ctctgtatta ggctcccaac ccttggtctt cttctgcatc
      361 acgggaccat tggagggaaa gtttacccct ctgacttgga cgtggtaaac gagacagcca
      421 tcctcaaggc tggtgtcctg catgacggtg acgactccac catcttcgta tctcgtaact
      481 ctttcccaag taaaaccctc aggaaaggac tgtttaaaga aatcaggaat gcctttcggg
      541 tacttgataa aagtacggct gccatacatg aacgacgtgg caagaatgtc aaaggcaaat
      601 ggcaggggtc ctccctcgat gactttgatc ctcatggttt gagttcccat gtacggattg
      661 ccttctcctt cacctgtgca tttgaattgg tggccgttga ccgaaccttc catgaccacc
      721 ttcatacgca tattttcctt gatcagctct tcgcccttag acaccatgct ttgccttcgt
      781 ttatcttgcc tgctcatttt ttagtatatt cttcgaaga
//
