LOCUS       sequence-77471-e         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Mank M, Santos AF, Direnberger S, Mrsic-Flogel TD, Hofer SB, Stein 
            V, Hendel T, Reiff DF, Levelt C, Borst A, Bonhoeffer T, Hubener M, 
            Griesbeck O
  TITLE     A genetically encoded calcium indicator for chronic in vivo 
            two-photon imaging.
  JOURNAL   Nat Methods. 2008 Sep;5(9):805-11. doi: 10.1038/nmeth.1243.
  PUBMED    19160515
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-09"; volume: "5"; issue: 
            "9"; pages: "805-11"
COMMENT     Sequence Label:  TN-XXL pRSETB
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(37..495,496..498,499..501))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     176..195
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     435..456
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(562..583)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cttgaagttg gccttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
       61 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      121 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      181 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      241 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc acatcaggcc
      301 gtagcccagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
      361 gaacttcagg gtcagcttgc cgttggtggc atcgccctcg ccctcgccgc gcacgctgaa
      421 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
      481 ctcctcgccc ttgctcacca tgctgccgcc ggtaccgccc ttgtacagct cgtccatgcc
      541 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      601 ggggtctttg ctcagcttgg actggta
//
