LOCUS       sequence-77482-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Thestrup T, Litzlbauer J, Bartholomaus I, Mues M, Russo L, Dana H, 
            Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, 
            Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger
            C, Griesbeck O
  TITLE     Optimized ratiometric calcium sensors for functional in vivo imaging
            of neurons and T lymphocytes.
  JOURNAL   Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773.
  PUBMED    24390440
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773."
COMMENT     Sequence Label: Twitch-4 pcDNA3
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(72..584,585..587,588..590))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     265..284
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     524..545
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(651..672)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atagaatagg gccctctaga tgcatgctcg agcggccgcc agtgtgatgg atatctgcag
       61 aattcttagt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc gttcttctgc
      121 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
      181 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
      241 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
      301 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
      361 tcggggtagc gggcgaagca catcaggccg tagcccaggg tggtcacgag ggtgggccag
      421 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gttggtggca
      481 tcgccctcgc cctcgccgcg cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
      541 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat gctgccgccg
      601 gtaccgccct tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      661 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctggtagctc
      721 aggtagtggt tgtc
//
