LOCUS       sequence-77486-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression vector for MBP-SLIRP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   McKay DB, Xi L, Barthel KK, Cech TR
  TITLE     Structure and function of steroid receptor RNA activator protein, 
            the proposed partner of SRA noncoding RNA.
  JOURNAL   J Mol Biol. 2014 Apr 17;426(8):1766-85. doi: 
            10.1016/j.jmb.2014.01.006. Epub 2014 Jan 30.
  PUBMED    24486609
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2014.01.006"; journalName: "J Mol Biol"; date: 
            "2014-04-17- 17"; volume: "426"; issue: "8"; pages: "1766-85"
COMMENT     Sequence Label: SLIRP.HMG6.wt.fl
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             381..398
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(451..469)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      465..512
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(636..655)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggccatagcg gcggcggcgg cggcggccat atggctgcgt cggctgcccg tggtgctgct
       61 gccctgcgtc gctcgatcaa tcaaccggtg gcgttcgttc gtcgtatccc gtggaccgcg
      121 gccagctctc agctgaaaga acattttgca caattcggcc acgtccgtcg ctgcattctg
      181 ccgtttgata aagaaaccgg tttccatcgt ggcctgggtt gggttcagtt tagtagcgaa
      241 gaaggcctgc gcaacgcgct gcagcaagaa aatcacatta tcgacggtgt gaaagttcaa
      301 gtccacaccc gtcgtccgaa actgccgcaa accagcgatg atgaaaaaaa agacttctag
      361 ggtaccgcgg ccgcctcgag caccaccacc accaccactg agatccggct gctaacaaag
      421 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      481 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
      541 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      601 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      661 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      721 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
      781 tagtgggcca tcgccctgat agacggtttt t
//
