LOCUS       sequence-77487-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression vector for MBP-SRAP(1-236) fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   McKay DB, Xi L, Barthel KK, Cech TR
  TITLE     Structure and function of steroid receptor RNA activator protein, 
            the proposed partner of SRA noncoding RNA.
  JOURNAL   J Mol Biol. 2014 Apr 17;426(8):1766-85. doi: 
            10.1016/j.jmb.2014.01.006. Epub 2014 Jan 30.
  PUBMED    24486609
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2014.01.006"; journalName: "J Mol Biol"; date: 
            "2014-04-17- 17"; volume: "426"; issue: "8"; pages: "1766-85"
COMMENT     Sequence Label: hSRAP.HMG6.wt.Dlink.a1-236
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cggtaccaaa actgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa
      121 cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga
      181 gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga
      241 cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga
      301 aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg
      361 ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa
      421 caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga
      481 actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg
      541 gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat
      601 taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct
      661 gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa
      721 taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag
      781 caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat cc
//
