LOCUS       sequence-77488-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Thestrup T, Litzlbauer J, Bartholomaus I, Mues M, Russo L, Dana H, 
            Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, 
            Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger
            C, Griesbeck O
  TITLE     Optimized ratiometric calcium sensors for functional in vivo imaging
            of neurons and T lymphocytes.
  JOURNAL   Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773.
  PUBMED    24390440
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773."
COMMENT     Sequence Label: Twitch-1 pcDNA3
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(73..585,586..588,589..591))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     266..285
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     525..546
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(652..673)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcttag tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      121 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
      181 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      241 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      301 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
      361 gtcggggtag cgggcgaagc acatcaggcc gtagcccagg gtggtcacga gggtgggcca
      421 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgttggtggc
      481 atcgccctcg ccctcgccgc gcacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      541 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgctgccgcc
      601 ggtaccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      661 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actggta
//
