LOCUS       sequence-77524-e         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-CymR
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..612
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
ORIGIN
        1 atgagcccga aacgtcgtac ccaggcagaa cgtgcaatgg aaacccaggg taaactgatt
       61 gcagcagcac tgggtgttct gcgtgaaaaa ggttatgcag gttttcgtat tgcagatgtt
      121 ccgggtgcag ccggtgttag ccgtggtgca cagagccatc attttccgac caaactggaa
      181 ctgctgctgg caacctttga atggctgtat gagcagatta ccgaacgtag ccgtgcacgt
      241 ctggcaaaac tgaaaccgga agatgatgtt attcagcaga tgctggatga tgcagcagaa
      301 ttttttctgg atgatgattt tagcatcagc ctggatctga ttgttgcagc agatcgtgat
      361 ccggcactgc gtgaaggtat tcagcgtacc gttgaacgta atcgttttgt tgttgaagat
      421 atgtggctgg gtgtgctggt gagccgtggt ctgagccgtg atgatgccga agatattctg
      481 tggctgattt ttaacagcgt tcgtggtctg gcagttcgta gcctgtggca gaaagataaa
      541 gaacgttttg aacgtgtgcg taatagcacc ctggaaattg cacgtgaacg ttatgcaaaa
      601 ttcaaacgtt aa
//
