LOCUS       sequence-7755-ex       10038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Controlled expression of transgenes introduced by in vivo 
            electroporation.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 
            10.1073/pnas.0610155104
  PUBMED    17209010
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2007-01-16- 16"; 
            volume: "104"; issue: "3"; pages: "1027-32"
COMMENT     Sequence Label: pNdrg4-DsRed
FEATURES             Location/Qualifiers
     source          1..10038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             5262..5361
                     /estimated_length=100
     regulatory      6192..6201
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6198..6872
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(6378..6398)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6790..6813
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             6882..6971
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     primer_bind     complement(7062..7081)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7127..7182
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7181..7200)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7541..7557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7541..7557)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7554..7576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7565..7581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7589..7595,7596..7613,7614..7619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7634..7655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7714..7909
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7760..7895
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7915..8049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7952..7971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8006..8025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8117..8134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8288..8876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8368..8387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9047..9838,9839..9907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9670..9689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9908..10012)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agtgtccttg gtacaagcag atcttggtcc cctggggaca
       61 gcagccctgc cctgtgatgt cctcacccgg cagcttcaga acaaggccca acctcgtact
      121 cccttcctcc tagggcttct gctccacgac gtgaccatgg cagggctgca ggagctgcga
      181 tttcctgagg agaagcccct gctccgcggc caggatgcca ctgagatggt gagtcgcccc
      241 ttccaggtcc tggggctgag cgccaccttg tggtgaaatt ggaaattgtg tgttcttgtc
      301 tttcctgttg tcatggaccc tccctgtgag agctcagagc tacccagaac tgatgttcat
      361 tccctgagaa atgtgtcagg gccagccagc ctgagtgccc agcccatgtc gctgggtatg
      421 gtccccgctc accttgcttt gcagctggga gaggcaggag cccagagcct tccaccttta
      481 gctccgtgtt tcctttccac ccaccttcag cttccttcca gaagaagccg caaggattgg
      541 gatcggatgg atggcttagc cagcgctctg gggaatccca gcacatgaga ccatagccct
      601 gcccctgaga gcatggtctg tccttccttt aacacataac tcccaggtta gttgagggta
      661 gctcgtgagg ccagaagtga acgtatttgg caagctcttc aacccaggtt catcctcctg
      721 ctaggatgcc tgcgggaacc tccttcctgc atccccactt ttctccttgc gcagtacaac
      781 cctttaaaga cacacaataa tagtcctatg cagccctatc tgttgtaatg gggagaccct
      841 ccccagagct cagaaccacc ctaaactgac tctccaatgt tcattcttga ggctggggga
      901 ttgccttctc tttaccctgc ctccctcctt ccctgcctcc ttgaaccttt gagcgtatct
      961 gccatattcc caggtgcttc ctctgctgga agacattagc ccaggaatgc atagaacagg
     1021 ctgtcacacc ctgaccctga agcccgggct ggcctgttct gtgcccaggt ctctccagcc
     1081 tcctgcagca cattgctatc cctttgtcct gactcacagt cctttgcttc cagttcgtgg
     1141 caacctcctt cttccttctg ctctacccag cctctcactt cctgccaccg gggccatagc
     1201 ttgcctctct ggagtttgga tctgtgttgc agtctcacga ggaggtctca gtccttgagt
     1261 cagcacaagg ttctcactgg gtacccagtg gcctctgctt ttcccaaagg ggcaaaaaaa
     1321 aaaaaaaaaa aaaaaaaaaa accacccagt gcagcctcca cgtgactccc agcaaagtcc
     1381 ttgaggtgtc agctagcttt accttacatt gtctttggga gctgccgttt ctcgacctgt
     1441 gatgctggca gagtctcata gcaccctgag tgcagtgcct gcctgacctc tatgttctga
     1501 gtggctgtag tttgccaggt acacattctt gttatcccac gatccaccct ccccccaccc
     1561 cgcccccata gccatggtat ccacagcggg gggggggggg gggggggctt cagttagtga
     1621 gatagaagca gcccaggaaa agcatctgta cacacttttt ttttccttgc ctttattccc
     1681 ttaacagcag agtatagcaa ctatttccat gacatatgtc ttctagaagg tgtgttctgg
     1741 gcaacccaga gccgatttaa attgtgtgag aggagaatcg taggctctgg gcaaagaaaa
     1801 tgccatctca gagaagtggg ctctggtacc tacaaaagac tctgtaacca atgcagaggc
     1861 gggagggtga ctatattcta tatacatctc gacctctata agccacttta gactagctct
     1921 ccctgaggac tgcagagtcc tgggatctgt aaggtgctgc ctgctccctc tctgcacccc
     1981 gctggtctcc acactctcct gggctttcct agcatcccga ccctctcctg ccctttgttt
     2041 tgatctcata tatacctcct atggcgccca gggattcatg ttggattatt tctttctgct
     2101 atctcttggc ttgtggaccc catccctccc acactcctat ctcctgagcc cccaacatcc
     2161 cgagagcaca ctgcacaccc taccattatc tgcctcatcc tttctgtcag attttgtttt
     2221 taacagaacc gcctccctcc ttccccgatg taattacctt cccacttgct cttctgtttg
     2281 gtgagcttgg tgaagggctc cgtcatcagt gaacagccct acccccttct gccagcagcc
     2341 ccccttcctg aggggttccc aaagtggctg ccctccctcc cttggcccct gtcgttcctc
     2401 ccaggccttg gaagaatcag tgggtctcac tacagagctg aggctggcct tggacttaag
     2461 atccccctat tccaccctcc tgaacgctgg aaatacctgc agtgctatgt gggtgtgttc
     2521 tgagccgtag gcccctcatt tgtgaaggca ggttgttcat ttgatcagcc tgtgttcagg
     2581 agcccactct agtccagcct ttacatgacc agatacggtc ccctcctcaa ggtaccagag
     2641 gcaggacagg cagggatcaa agatgcatgc cactgacagt ttgacattga cccaaggttt
     2701 gagacagtaa agtgggcagt gtggctggcc ttgacaacac ctaatatcca taggtcagga
     2761 cagtgaacag gcctcagctt acccacccag ccctgggcgt gacagtcata gtgcttcttg
     2821 gctccttcat cctcttccct tatgtgtaat gaggcagaga tctcagtgct ttctgcctgt
     2881 ggtgcagctc tgagtctccc cgttaaccca gaagatagaa atccttgcac cgggtaacct
     2941 gtgagtcact tctgttagca tgaatgtaga ctggggatca gaagatgggt ctgctctgtg
     3001 cagagccctg ggagctgggt taacttgccc ccttttgttt gttctcctta ggataaccct
     3061 gacgccttcc tctcaattgt ggacacagac tggaaggtag gttggggttc agcctctcaa
     3121 atatgggacc catatctgtc tggacttctc tcccactggg ctgggtccta gaggagagta
     3181 atagcctgtc catgtgtccc agtctctggt ggtgagggca gggccacctc acggcctaat
     3241 gacccccacg gctcacagca gctccccctg cccagtgaag gggttggacc ctctgtgaac
     3301 aggacagttg gaaaggaagc agaggcctcc ggcaatgagt gtgcagaaag gagcgaggga
     3361 tgctgtcatc tgaatcagca ttttcctccg gtgaaagcta ggttgactgt gaaataactc
     3421 ttgtttccat agcagctgtt gcctggacaa ccatgtcccg gttagtttga tgccaaaggt
     3481 accatgaaaa tggaactcgc tctttgtttt tattccttca catgatgatt ttgctcactt
     3541 ggccagctat cccccctgcc tcgagtccct taggaacggt ttatttcctg gcaccagaat
     3601 atgtctaaca gcaagcatag gatggagagg gaaacggggt tattgggttc ccagacgcca
     3661 ctggccaccc aaataggaca aaccccctcc tccctgctgg atggaagtca cctccaggcc
     3721 acatccttaa atagataagc gttgggcaac tagggcaact agatggtggg accctggttc
     3781 tcaagacctc ttacttggtg aaggacagca aaggaatcct cctgtcactc tgggagcact
     3841 acatagaggc aggttttgtt cggttttcct ttttgcagaa aagcagccac aaaagatggc
     3901 tgttacctgg gccacagccg gggagccatc ttcatcccca gcttcacagc ggcaggcatt
     3961 gtcactgtcg ctggccttac agggtcagcg cgggtgacag aagcaagaca gagaaactag
     4021 aaaaatacaa gagaccggat tctaggtcca gcagtgtcca ccccacccct gccatcctgc
     4081 tcccagactg ctcgcctcgt acgaggggat ggtgtccaag ggtccagtgc tcagacaccc
     4141 ccagtgacca tgacaaggca cagccagcca cagcacatag ccgtagcaga ctgactgatg
     4201 tggatcctgg tgcagtggct tccagctgtg aagtagggca gcttgccacc tgtctgaaga
     4261 gtcgggtgct gagtgagcta atgcactcag gtagggtcta gcattcaggc actaacacag
     4321 tgtctgttcc cttagtggtt gtgtgtgagt ctggaccgct gaggatgtaa gttgcgctgc
     4381 tctcgcctga gtgagaaagc acgctcactt cgggggctct ttgtgctttc tcccaggctt
     4441 ctgtgtctca gaagcacaga ataaaaaaaa aaccttgccc ccaggactct tcagacagcc
     4501 gctgggcagg cctggcctcg ggtcttaggg tttgtagcca gtgagctgaa cagaaacagg
     4561 tagtgtggaa gaggccagtg ttgggtctgc gtttcctttg gagccaggga aggagaagcc
     4621 actcagtagg tagtcaggga gtggatgtgg attggtcagg gcccacgggg agtgggagtt
     4681 ggtggcagga cctttctgtt cccttgagag agtcatggga gctctttagg gacagagcag
     4741 gacaggaggg ccagggacac actgttccat gactcccgtt tgggagtgga atggtgaagc
     4801 gtggcacaga gaaagctggt ggtaccccaa gacagaggct gctactaggc ctagtgccca
     4861 tgactactgc aagtcaggtc acatgacttg caaaacagct cagcagacac ctgggtgggt
     4921 ggggagctgg cccccttccc atggaaccca ccagggtgca gggtggtaca gtcctccatg
     4981 gagaagacaa taggggcaca gacacccagc tcccaccatg ttcctgtcgc tcgggttctc
     5041 ttagccctgg ggctgcctcc tctgtcacct ccctggcctc tccttttggc actgctcctc
     5101 tctgtgtgcc tcagaccagc ttctcaccct gagcctcact gcccagtaga tacatcgtgt
     5161 agccttgggg atcttctcca cagacttcgg agctggactc catactgact ggctctaaac
     5221 acagggctgg ttggagtggg gggggggcgg ggggggggaa tnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nttcgggggg ggggaatgca agcttgccag cttctctcct
     5401 tccctcctat aggagtgtga ggttagggag ggccccgccc gcctgctcct ttgcttctct
     5461 gcccctgcag tcgacccagc cctgggcatc cctataggta gggtgggcaa tgcccaaggg
     5521 gagagacagg ccagtagcct gccttcaagg acagtaagtg acttaggctg tcatcttgct
     5581 gggatgggtg ggcttgagag tcgggtcctc cagatcctgg ggcaagggcc ctcccaagat
     5641 cttggtactt ccaagggaat agagccaagt gccggggcag gtctgcacac ccagcttctc
     5701 ctcgggtcta gccctgcatc ggtatacatc ggtatatgta tacatcggta tacatatcgg
     5761 tgagaggggc gcctctgtgt gcaggggtac ctgtgtggcc ttagctgttg ctccccggaa
     5821 gccacataca gctgtaccta atggatggtg ccagggtgct aagcaccctt gagcggcaga
     5881 agctggggac cctcaggatg ctgtgtggct cgggagggag gggctgaagt gaggcggggt
     5941 gaacgagtac ctcagaagcc aattggagcc aggtttgtct cctaacagcc aatgcgggag
     6001 cgagggctcc gggtgagcaa gtggctgcgc agaaaccttg cagggaggcg ggaccagcct
     6061 gggcagggcc aggatctgtg tataaaacca ctggagctct accccaccac agagccgaag
     6121 tctcccaccc aggctgcccc ctacctccag accgagtgac tcaggctttt gttgaattcg
     6181 atatctgagc cgccaccatg gcctcctccg agaacgtcat caccgagttc atgcgcttca
     6241 aggtgcgcat ggagggcacc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     6301 gccgccccta cgagggccac aacaccgtga agctgaaggt gaccaagggc ggccccctgc
     6361 ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc
     6421 accccgccga catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     6481 gcgtgatgaa cttcgaggac ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg
     6541 acggctgctt catctacaag gtgaagttca tcggcgtgaa cttcccctcc gacggccccg
     6601 tgatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     6661 gcgtgctgaa gggcgagacc cacaaggccc tgaagctgaa ggacggcggc cactacctgg
     6721 tggagttcaa gtccatctac atggccaaga agcccgtgca gctgcccggc tactactacg
     6781 tggacgccaa gctggacatc acctcccaca acgaggacta caccatcgtg gagcagtacg
     6841 agcgcaccga gggccgccac cacctgttcc tgggaccggt ggagcagaaa ctcatctctg
     6901 aagaagatct ggaacaaaag ttgatttcag aagaagatct ggaacagaag ctcatctctg
     6961 aggaagatct gtgatagcgg ccgcactcct caggtgcagg ctgcctatca gaaggtggtg
     7021 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     7081 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     7141 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     7201 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     7261 atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc
     7321 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     7381 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     7441 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     7501 aagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7561 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7621 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7681 tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt
     7741 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7801 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7861 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg
     7921 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7981 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8041 gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8101 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8161 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8221 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8281 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8341 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8401 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8461 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     8521 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8581 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8641 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8701 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8761 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8821 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8881 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8941 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9001 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9061 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9121 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9181 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9241 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9301 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9361 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9421 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9481 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9541 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9601 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9661 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9721 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9781 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9841 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9901 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9961 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10021 cccgaaaagt gccacctg
//
