LOCUS       sequence-77637-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression vector for MBP-SLIRP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   McKay DB, Xi L, Barthel KK, Cech TR
  TITLE     Structure and function of steroid receptor RNA activator protein, 
            the proposed partner of SRA noncoding RNA.
  JOURNAL   J Mol Biol. 2014 Apr 17;426(8):1766-85. doi: 
            10.1016/j.jmb.2014.01.006. Epub 2014 Jan 30.
  PUBMED    24486609
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2014.01.006"; journalName: "J Mol Biol"; date: 
            "2014-04-17- 17"; volume: "426"; issue: "8"; pages: "1766-85"
COMMENT     Sequence Label: SLIRP.HMG6.wt.fl
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcacg
       61 gtaccaaaac tgaagaaggt aaactggtaa tctggattaa cggcgataaa ggctataacg
      121 gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc accgttgagc
      181 atccggataa actggaagag aaattcccac aggttgcggc aactggcgat ggccctgaca
      241 ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg ttggctgaaa
      301 tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat gccgtacgtt
      361 acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca
      421 aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac
      481 tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc
      541 cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacgacatta
      601 aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga
      661 ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata
      721 aaggcgaaac agcgatgacc atc
//
